WebPairwise Structure Alignment; Symmetry Resources in the PDB; Structure Quality ; Grouping Structures; PDB Citation MeSH Network Explorer ; PDB Statistics; EPPIC Biological Assemblies ; External Data and Resources ; Integrated Resources ; Additional Resources ; Download . Coordinates and Experimental Data; Sequences; Ligands WebOverall structure of human ACAT2 holoenzyme (A) Either nevanimibe or PPPA inhibits the activity of ACAT1 and ACAT2 in vitro. In the activity assays, ACAT activity was measured …
3D View: 7N6R - rcsb.org
Web7N6R: Structure of nevanimibe bound human ACAT2. You are using a web browser that we do not support. Our website will not work properly. WebSep 1, 2024 · Here we report a cryo-electron microscopy structure of human ACAT1 in complex with nevanimibe7, an inhibitor that is in clinical trials for the treatment of … discord.js v13 slash command handler github
Structure of nevanimibe-bound tetrameric human ACAT1,Nature
WebMemProtMD simulation of Structure of nevanimibe bound human ACAT2 in a lipid bilayer at both coarse-grained and atomistic respresentation, including both file download and analysis. Classification based on PDB 7n6r. WebHandling Instructions Nevanimibe (PD-132301) is an orally active and selective acyl-coenzyme A:cholesterol O-acyltransferase 1 ( ACAT1) inhibitor with an EC50 of 9 nM. Nevanimibe inhibits ACAT2 with an EC 50 of 368 nM. Nevanimibe induces cell apoptosis and has the potential for adrenocortical cancer . For research use only. For 200 kV cryo-transmission electron microscopy (cryo-TEM), the nevanimibe-bound ACAT1 sample was crosslinked by the addition of 0.1% glutaraldehyde (Sigma-Aldrich) and incubated at room temperature for 30 min. The reaction was then terminated by the addition of 50 mM Tris, pH 8.0 at room … See more No statistical methods were used to predetermine sample size. The experiments were not randomized and the investigators were not … See more The cDNA of human ACAT1 (GenBank: BC028940.1) was cloned into pEG BacMam with a C-terminal Flag-tag. The protein was … See more The nevanimibe-bound ACAT1 sample (4 mg ml−1 native protein, not cross-linked) was applied to Quantifoil R1.2/1.3 300 or 400 mesh Au holey carbon grids (Quantifoil). The grids … See more ACAT activity was measured by monitoring the CoA released from an acyltransferase-mediated reaction21. The sulfhydryl (-SH) group of CoA can react with CPM and the resulting highly fluorescent product is … See more four fish inn jensen beach