Fitc rabbit anti- active caspase-3
WebJan 9, 2024 · Caspase-3 expression in HCT-116 cells was quantified through flow cytometry by using the FITC rabbit anti-active caspase-3 IgG antibody. A significant increase in the caspase-3 FITC-MFI was observed in HCT-116 cells treated with AgNPs (1936, 1.5-fold increase) and those treated with cisplatin (44 μg/mL) (4551, 4.5-fold increase), as … WebMay 3, 2000 · The presence of a low amount of active caspase 3 in the treated viable cells was confirmed in situ with PE-conjugated anti–active caspase 3 antibodies. The same antibody was used in combination with FITC-annexin V and CD45–PC5 to study caspase 3 activation in acute leukemia blast cells after in vitro DNR and IDA treatment.
Fitc rabbit anti- active caspase-3
Did you know?
WebAnti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow. Immunocytochemistry Analysis: A … WebAug 20, 2013 · The caspase activity was assessed by the FITC rabbit anti-active caspase-3 (BD Pharmingen, San Diego, CA, USA). The cells were treated with CuE of 0, 1.25, 2.5 and 5 μM for 24 h. The caspase activity was detected and inspected by the FACSCalibur flow cytometry. Data was analyzed using WinMDI 2.8 free software (BD, …
WebActive caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This … WebHuman Active Caspase-3 Fragment. Validations. None available. Applications. Enzyme-linked immunosorbent assay (ELISA) Immunohistochemistry-frozen tissue (IHC-Frozen) …
WebFITC Rabbit Anti- Active Caspase-3 Product Information Material Number: 559341 Alternate Name: CPP32; Yama; Apopain Size: 100 tests Vol. per Test: 20 µl Clone: C92 … WebThis Caspase-3 Antibody (31A1067) - (Pro and Active) was developed against full-length recombinant human caspase-3 protein. The antibody detects both pro Caspase-3 (~32 …
WebJan 6, 2024 · caspase-3 Antibody (E-8) is a mouse monoclonal IgG 2a κ caspase-3 antibody, cited in 729 publications, provided at 200 µg/ml. raised against amino acids 1-277 representing the full length precursor form of caspase-3 of human origin. caspase-3 Antibody (E-8) is recommended for detection of caspase-3 and full length procaspase-3 …
http://pufei.com/static/product/bd/550/1505231617e995b8.pdf flow khail fongWebSep 23, 2014 · The caspase activity was assessed by the FITC rabbit anti-active caspase-3 (BD Pharmingen, USA). The cells were treated with CuE of 0, 0.625, 1.25 and 2.5 μM for 24 hours. flow kimonos hempWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) Research with confidence – consistent and reproducible results with every batch. Long-term and … green ceramic drum tableWebCellular detection of the apoptotic markers caspase-3, bcl-2 and Bax activity was determined by FCM employing the fluorescein isothiocyanate conjugated monoclonal antibodies (caspase 3 FITC, Rabbit anti- active caspase 3 cat. No, 559341), (PE Hamster Anti-Mouse Bcl-2 Set Cat No. 556537) and (Bax Monoclonal Antibody (6A7) Cat No. … green ceramic elephant planterWebJul 20, 2024 · To measure the level of cleaved caspase-3, cells were fixed with 75% (v/v) ethanol in PBS, permeabilized with 0.25% (v/v) Tween 20 in PBS and stained overnight with a rabbit antiserum specific for ... flow kimonos hemp beltWeb25546-1-AP targets cleaved-Caspase 3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for … green ceramic floor tileWebCaspases play a crucial role in cell apoptosis or programmed cell death. 70 Effector caspase 3 is responsible for the actual cleavage of cellular components during apoptosis. Hence, in the present study, caspase-3 level was estimated using FITC rabbit anti-active caspase-3 IgG antibody assay kit (BD Biosciences, catalogue no. 559341). flowkinetics